Amniotic fluid heat shock protein 70 concentration in preterm premature rupture of membranes

Czech version

Authors: M. Kacerovský ¹; J. Tošner ¹; C. Andrýs ² ; M. Drahošová ²; L. Plíšková ³; M. Főrstl ⁴; H. Hornychová ⁵
Authors‘ workplace: Lékařská fakulta Hradec Králové, Univerzita Karlova, Praha Porodnická a gynekologická klinika FN, Hradec Králové, přednosta doc. MUDr. J. Tošner, CSc. ¹; Ústav klinické imunologie a alergologie FN, Hradec Králové, přednosta prof. RNDr. J. Krejsek, CSc. ²; Ústav klinické biochemie a diagnostiky FN, Hradec Králové, přednosta prof. MUDr. V. Palička, CSc. ³; Ústav klinické mikrobiologie FN, Hradec Králové, přednosta doc. RNDr. V. Buchta, CSc. ⁴; Fingerlandův ústav patologie FN, Hradec Králové, přednosta prof. MUDr. A. Ryška, Ph. D. ⁵
Published in: Ceska Gynekol 2009; 74(2): 85-91
Category: Original Article

Overview

Objective:
The purpose of this study was to determinate the changes of amniotic fluid HSP 70 concentrations in patiens with preterm premature rupture of the membranes, and in the presence of intraamniotic infection and histological changes of inflammations.

Design:
Prospective study.

Setting:
Department of Obstetrics and Gynecology Medical Faculty Charles University Hradec Králové.

Methods:
We studied 30 women between 24 and 36 weeks of gestation with preterm premature rupture of the membranes. Samples of amniotic fluid were collected by transabdominal amniocentesis. These patients were divided into 2 groups. In group 1 were patiens with intraamniotic infection. In group 2 were patiens without intraamniotic infection. Among 76% (35/30) patients placenta were collected and assessed for presence or absence acute inflammatory lesions. HSP70 concentration in amniotic fluid were determined using a sensitive and specific diagnostic kit Hsp 70- ELISA kit manufactered Assay Desings, USA.

Results:
There was no significant difference in the median amniotic fluid HSP70 concentration between patients with preterm rupture of the membranes with IAI and without IAI (patients with IAI: median 5.12 ng/ml, range 3.01-90.37 ng/ml vs. patients without IAI: median 4.68 ng/ml, range 0.58-84.28 ng/ml; p=0.56). There was no significant difference in the median amniotic fluid HSP70 concentration between patients with preterm rupture of the membranes with presence and absence histological of acute inflammatory lesions in the placenta and membranes(patients with presence: median 6.97 ng/ml, range 2.61-90.37 ng/ml vs. patients with absence: median 4.63 ng/ml, range 0.58-84.28 ng/ml; p=0.68).

Conclusion:
Intraamniotic levels HSP70 were not associated with intraamniotic infection and acute inflammatory lessions in the placenta and membranes.

Key words:
HSP70, intraamniotic infection, chorioamnionitis, preterm premature rupture of the membranes.

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