IgG antibodies against laminin-1 in serum and in peritoneal fluid in patients with decreased fertility

Czech version

Authors: R. Červená ¹; K. Bibková ¹; Z. Mičanová ¹; B. Šedivá ²; Z. Ulčová-Gallová ¹
Authors‘ workplace: Gynekologicko-porodnická klinika LF UK a FN Plzeň, přednosta doc. MUDr. Z. Rokyta, CSc. ¹; Katedra matematiky, Fakulta aplikovaných věd, Západočeská Univerzita, Plzeň, vedoucí prof. RNDr. P. Drábek, DrSc. ²
Published in: Ceska Gynekol 2009; 74(3): 188-192

Overview

Objective:
To study of IgG antibodies against laminin-1 (IgG-a-Ln-1) in patients with decreased fertility, especially with endometriosis and with diagnosis of polycystic ovaries.

Design:
Prospective study.

Setting:
Special Consultation for Immunology of Reproduction and Research Laboratories for Reproductive Immunology, Department of Gynaecology and Obstetrics, Medical School of Charles University and Faculty Hospital, Pilsen.

Methods:
Commercial ELISA kits for detection of IgG antibodies against laminin-1 in serum (S) and peritoneal fluid (PF) in 120 patients after diagnostic laparoscopy, and in serum in 277 patients after 2-3 in vitro fertilizations and with diagnosed endometriosis.

Results:
Only 16 positive results in IgG-a-Ln-1 antibodies (more than 15 IU/ml ) were detected in sera and peritoneal fluids in the group of 120 women after laparoscopy.

We have not detected any serum IgG-a-Ln-1 positivity in the group of 277 patients after 2-3 unsuccesfull IVF and with anamnestical date about endometriosis together.

Conclusion:
Increased levels of IgG-a-Ln are in particular found in endometriosis Iľ-IIľ and in diagnosis of polycystic ovaries, in female diseases also related to autoimmune process.

Key words:
infertility, endometriosis, polycystic ovaries, IgG antibodies against laminin-1.

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