Umbilical cord blood soluble Toll-like receptor 2 in pregnancies complicated by preterm premature rupture of membranes

Czech version

Authors: E. Mitášová ¹; M. Kacerovský ²; J. Krejsek ¹; H. Hornychová ³; C. Andrýs ¹
Authors‘ workplace: Ústav klinické imunologie a alergologie LF UK a FN, Hradec Králové, přednosta prof. RNDr. J. Krejsek, CSc. ¹; Porodnická a gynekologická klinika LF UK a FN, Hradec Králové, přednosta doc. MUDr. J. Špaček, Ph. D. ²; Fingerlandův ústav patologie LF UK a FN, Hradec Králové, přednosta prof. MUDr. A. Ryška, Ph. D. ³
Published in: Ceska Gynekol 2013; 78(4): 365-372

Overview

Objective:
To determine whether umbilical cord blood concentrations of soluble Toll-like receptor (sTLR2) is of value in the diagnosis of histological chorioamnionitis (HCA) and funisitis in pregnancies complicated by preterm premature rupture of membranes.

Design:
Retrospective study.

Setting:
Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Kralove, Department of Clinical Immunology and Allergy, Department of Obstetric and Gynecology.

Methods:
Eighty six women with PPROM between gestation ages 24 and 36 weeks were included in the study. The samples of the umbilical cord blood were taken from the clamped umbilical cord immediately after delivery of the newborn. The placenta, fetal membranes and umbilical cord were evaluated for the presence of inflammatory changes. The concentrations of sTLR2 in the umbilical cord blood were measured by ELISA method.

Results:
Women with HCA did not have different umbilical cord blood sTLR2 levels than women without HCA (with HCA: median 7.6 ng/mL, interquartile range [IQR] 5.1 – 12.3 vs. without HCA: median 8.0 ng/mL, IQR 6.0 – 9.4; p = 0.79). No differences between women with and without funisitis were found (median 7.2 ng/mL, IQR 5.5 – 22.3 vs. without funisitis: median 7.9 ng/mL, IQR 5.2 – 10.5; p = 0.31).

Conclusion:
Umbilical cord blood sTRL2 levels are not affected by the presence of either HCA or funisitis in pregnancies complicated with PPROM.

Keywords:
funisitis – histological chorioamnionitis – innate immunity – preterm premature rupture of membranes – sTLR2

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