Meiotic spindle as a marker of optimal time for oocyte vitrification – presentation of successful infertility treatment and literature review

Czech version

Authors: Jaromír Mašata ¹ ; Olga Teplá ¹; Simona Jirsová ¹ ; Anna Pšeničková ¹; Martina Moosová ¹; Eva Fajmonová ¹; Irena Kratochvílová ²
Authors‘ workplace: Centrum asistované reprodukce, Klinika gynekologie, porodnictví a neonatologie 1. LF UK a VFN v Praze ¹; Fyzikální ústav, AV ČR, Praha ²
Published in: Ceska Gynekol 2024; 89(6): 475-478
Category: Case Report
doi: https://doi.org/10.48095/cccg2024475

Overview

Aim: Within the in vitro fertilization (IVF) process, to evaluate the possibility of using the state of the meiotic spindle of oocytes as an indicator of maturity in order to optimize the timing of vitrification. Patients and methods: In the presented report, the cause of couple infertility was a combination of a 38-year-old female and 43-year-old male with azoospermia, which was an indication for oocyte vitrification. Oocyte polar bodies and optically birefringent meiotic spindles were visualized by polarized light microscopy and their states and relative positions were used as indicators of oocyte maturation, i.e. readiness for vitrification. Oocytes which had an angle between the polar body and meiotic spindle a ≤ 30° or had a poorly visible meiotic spindle were vitrified 4 hours post-meiotic spindle evaluation (8 hours after ovum pick-up). Results: After thawing, oocytes were fully maturated and prepared for intracytoplasmic sperm injection (ICSI). Following treatment with tamoxifen, vital sperm were retrieved from the patient’s partner, the oocytes were thawed and fertilized with the obtained sperm, with subsequent embryo transfer and delivery of a healthy baby at term. Conclusion: The meiotic spindle can be used as an oocyte maturation pointer in older women. After thawing, the oocytes were fully matured and ready for fertilization by ICSI.

Keywords:

oocyte – IVF – pregnancy – meiotic spindle – polar body

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ORCID autorů

J. Mašata 0000-0002-3898-6608

S. Jirsová 0000-0001-8891-8737

I. Kratochvilová 0000-0002-6633-9432

Doručeno/Submitted: 24. 9. 2024

Přijato/Accepted: 10. 10. 2024

prof. MUDr. Jaromír Mašata, CSc.

Centrum asistované reprodukce

Klinika gynekologie, porodnictví a neonatologie

1. LF UK a VFN v Praze

Apolinářská 18

120 00 Praha 2