Artificial activation of sperm motility in vitro

Czech version

Authors: P. Trávník ^1-3 ; M Ješeta ^1,4,5 ; R. Hűttelová ^1,6 ; R. Křen ^1,7 ; L. Landsmann ^1,8 ; A. Nesvadbová ^1,9,10 ; G. Tauwinklová ^1,2,11
Authors‘ workplace: Výbor Asociace reprodukční embryologie z. s. ¹; Repromeda s. r. o., Brno ²; IPVZ, Praha ³; Gynekologicko-porodnická klinika LF MU a FN Brno ⁴; FAPPZ ČZU, Praha ⁵; Cube IVF, Praha ⁶; GENNET s. r. o., Praha ⁷; UNICA Prague s. r. o., Praha ⁸; IVF Clinic, Olomouc ⁹; Přírodovědecká fakulta, UP v Olomouci ¹⁰; Ústav histologie a embryologie, LF MU, Brno ¹¹
Published in: Ceska Gynekol 2024; 89(2): 134-138
Category:
doi: https://doi.org/10.48095/cccg2024134

Overview

Aim: The sperm activation method is a modern methodological approach that is used more and more often in practice. The number of studies focused on methods of artificial activation of human sperm motility are constantly increasing. Standard sperm selection methods can fail in some cases, among other things, because very young sperm are isolated that have not yet completed their development. In these cases, artificial stimulation of their movement can have a positive effect and greatly facilitate and faster the process of selecting suitable sperm. Methylxanthines are most often used as activating agents. However, opinions on the safety of using these substances on sperm are not uniform. The aim of the thesis is to present current knowledge about artificial activation of sperm motility for in vitro fertilization and subsequent embryonic development. Methodology: Research of relevant literature in Web of Science, Scopus, PubMed/Medline databases. Results and conclusion: The literature analysis shows that this method is safe and effective in the selection of immotile spermatozoa. Scientific studies have been conducted to verify the safety and reliability of this method. The conclusion of these studies is the positive impact of this method of selection, especially in cases of sperm obtained from testicular tissue after method testicular sperm extraction. In these cases, the method of artificial sperm activation facilitated and accelerated the selection of sperm before intracytoplasmic sperm injection. Undamaged spermatozoa, which are immobile due to incomplete maturation, were activated.

Keywords:

spermatozoa – In vitro fertilization – motility – theophylline

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ORCID autorů

P. Trávník 0000-0002-2966-923X

M. Ješeta 0000-0003-1778-3454

R. Hüttelová 0009-0001-1877-1060

R. Křen 0009-0002-3534-7816

L. Landsmann 0000-0001-5984-1232

A. Nesvadbová 0000-0001-7854-4268

G. Tauwinklová 0009-0001-3024-6357

Doručeno/Submitted: 20. 10. 2023

Přijato/Accepted: 24. 10. 2023

prof. MUDr. Pavel Trávník, DrSc.

REPROMEDA s. r. o.